9^th International Conference on Biopolymers and Polymer Sciences November 19-20, 2018 Bucharest, Romania

Benny Danilo Belviso has completed his PhD from University of Bari Aldo Moro and Postdoctoral studies from Institute of Crystallography (CNR). He has worked at Nottingham University in the MRC project to study ADAMTS13 protein structure and currently he is the Research Fellow at Institute of Crystallography. He is involved in several international projects related to structural biology and materials for crystallization applications. He has published more than 20 papers in reputed journals.

 

Since 60 years, X-ray crystallography provides structural details of protein molecules, information that is crucial to unravel biological mechanisms at molecular level. Crystallography requires that sample is in crystal form. Getting such crystals at acceptable quality for crystallographic analysis is not trivial and strategies to make this process less expensive and time consuming are not available, still now. Advanced materials represent a turning point in this field because they can be exploited to control nucleation and growth step, making more effective the crystallization process. Our group is developing membranebased materials able to trigger protein crystallization also in conditions that are not fruitful by standard methods. Such materials have a great impact both in industry and academic studies because significantly reduce cost and time of the protein purification and crystallization process. We developed membrane-materials functionalized by hydrogel that proved ability in getting very stress-resistant crystals, which are suitable for structure-based drug design studies that require very harsh soaking conditions. This material, similarly to our metal oxide nanoparticle-functionalized membrane, significantly widens crystallization window and produce crystals having good diffraction quality. Membrane based materials are showing very effective in protein crystallization and to produce crystals having specific features. Our efforts are focusing now in functionalizing such materials by nanotemplate to crystallize very challenging proteins such as intact antibodies, and to develop membrane able to promote bio-mineralization and to enable polymorphs selection.

 

Ramin Zibaseresht is a Professor in Chemistry at Maritime University of Imam Khomeini in Noshahr and Adjunct Professor at Aja University of Medical Sciences in Tehran. He has completed his BSc in Chemistry from Shiraz University and his MSc in Inorganic Chemistry from Pune University. He has completed his PhD in Inorganic Chemistry from the University of Canterbury. He is currently the Head of Biomaterials and Medicinal Chemistry Research Centre in Tehran. He has published more than 50 papers in reputed journals and some international conferences and more than 10 books in the area of chemistry and 4 patents. He has been serving as an Editorial Board Member of some peer-reviewed journals, academic book publishers, member of more than 10 academic committees, organizing committees and academic boards of international conferences.

 

The synthesis and crystal structure of a novel polymeric silver(I)-iron(II) complex containing bridging ligand 4’-(4-(2,2,2-tris(1H-pyrazol-1-ido)ethoxymethyl)phenyl-2,2’:6’,2”-terpyridine (TPT) are described. The reaction of TPT with FeCl2.6H2O afforded a complex [Fe(TPT)2]Cl2 which in turn reacted with a range of silver salts such as AgNO3, AgClO4 resulted in the formation of hetero-metal complexes which were characterized using 1H NMR and ES-MS techniques. The reaction solution of the [Fe(TPT)2]Cl2 complex with molar equivalent of AgClO4 resulted in a solution with face needlelike crystals suitable for single X-ray crystallography. The complex was crystallized the triclinic space group, PÄ«. The smallest repeating unit of the complex contains an [Fe(TPT)Ag2(H2O)2](ClO4)3 unit. The Fe atom is coordinated by three nitrogen of terpyridine moiety from one TPT ligand and by three nitrogen of terpyridine moiety from another TPT ligand in an octahedral geometry fashion. While one Ag atom is coordinated by two nitrogen atoms of one pyrazolyl moiety from a TPT ligand and two nitrogen atoms of adjacent pyrazolyl moiety from another TPT ligand to generate a linear coordination polymer in a tetragedral geometry. The third nitrogen atom of the last pyrazolyl part is also coordinated to a silver ion which was itself coordinated to two water molecules through their oxygen atoms in a trigonal planar geometry. The shortest Ag-Ag distance is 5.337(1) Å within a TPT ligand. The Fe-Ag distances are 10.480(1) Å, 15.0637(1) Å within a unit cell. In vitro the study of the complex against some bacterial pathogens were also investigated.